ABSTRACT
<p><b>OBJECTIVE</b>The aim of this study was to explore the prevalent characteristics of HBoV1 and its co-infection.</p><p><b>METHODS</b>PCR was used to detect HBoV1-DNA (HBoV1) and other viruses. A multivariate logistic regression model was used to explore possibility of co-detected for related viruses.</p><p><b>RESULTS</b>The positivity rates in Nanjing and Lanzhou were 9.38% (74/789) and 11.62% (161/1386), respectively (P>0.05). The HBoV1 positive group was younger than negative group (P<0.05). Seasonal differences were noted, with a higher frequency of infection in December and July. HBoV1-positive children [72.34% (169/235)] were co-infected with other respiratory viruses. Multifactorial analysis showed no correlations between HBoV1 and the clinical classification, region, gender, age, or treatment as an outpatient or in a hospital. Correlations were identified between HBoV1 infections with ADV (OR=1.53, 95% CI 1.03-2.28), RSV (OR=0.71, 95% CI 0.52-0.98), and IFVA (OR=1.77, 95% CI 1.00-3.13).</p><p><b>CONCLUSION</b>Presence of HBoV1 in nasopharyngeal aspirates did not correlate with region or gender, although the prevalence of HBoV1 was higher in younger children. There were no correlations between HBoV1 and other variables, except for the season and ADV, RSV, or IFVA infections.</p>
Subject(s)
Child, Preschool , Female , Humans , Male , Acute Disease , China , Epidemiology , Comorbidity , DNA, Viral , Genetics , Human bocavirus , Genetics , Logistic Models , Multivariate Analysis , Parvoviridae Infections , Epidemiology , Virology , Prevalence , Respiratory Tract Infections , Epidemiology , VirologyABSTRACT
<p><b>OBJECTIVE</b>To sequence the complete sequence of bocavirus I with sequence independent single primer amplification (SISPA-PCR).</p><p><b>METHODS</b>To exclude the co-effection samples, all clinical samples of diarrhea cases were screened with special primers of rotavirus, astrovirus, adenovirus, calicivirus and bocavirus I. The virus were enriched through ultracentrifugation. Other nucleic acids, such as human and bacteria genomes, were degradated by DNase I and RNase. DNA of bocavirus was Amplificated with SISPA-PCR, then purificated, cloned and sequenced. The sequences were alighmented in nr with blastn and assembled with DNAstar.</p><p><b>RESULTS</b>A 4834bp sequence of bocavirus I were assembled.</p><p><b>CONCLUSION</b>SISPA-PCR is an economical and efficient technique for sequence a virus complete genome.</p>
Subject(s)
Humans , Base Sequence , Bocavirus , Genetics , DNA Primers , Genetics , Diarrhea , Virology , Genome, Viral , Parvoviridae Infections , Virology , Polymerase Chain ReactionABSTRACT
From November 2008 to January 2009, a sharp increase of diarrhea in children in Guangdong province appeared, we randomly collected 53 stool specimens from out-patient children with dirrhea in 3 major hospitals (Guangzhou City Children's Hospital, Shenzhen Baoan District Maternal and Child Health Hospital, First Affiliated Hospital of Shantou University). Rotavirus and calicivirus were screened by ELISA and RT-PCR. We found 29 cases of rotavirus infection with diverse serotypes. Only four cases were identified as calicivirus infection. The result indicated that rotavirus was a major pathogen of this high incidence of diarrhea from November 2008 to January 2009 in Guangdong Province.
Subject(s)
Child, Preschool , Humans , Infant , Age Distribution , China , Epidemiology , Diarrhea , Diagnosis , Epidemiology , Virology , Diarrhea, Infantile , Diagnosis , Epidemiology , Virology , Feces , Virology , Incidence , Reverse Transcriptase Polymerase Chain Reaction , Rotavirus , Classification , Genetics , Rotavirus Infections , Diagnosis , Epidemiology , Virology , SerotypingABSTRACT
Porcine sapoviruses (SaVs), which belong to the family Caliciviridae, have been considered potential zoonotic agents for human infection, and several cases have been reported in Asian countries. In this study, a total of 200 porcine fecal samples collected from Lulong county of China were tested. Among 200 samples, porcine sapoviruses were detected by RT-PCR in 17 samples (8.5%) showing their circulation in China. 14 out of 17 positive sapovirus strains were genetically related to the genogroup III (GIII) and were further divided into three different clusters or genotypes according to the phylogenetic analysis. In addition, the remaining three sapovirus strains belonged to GVII (one strain) and a potential novel genogroup (two strains) according to the phylogenetic analysis and the nucleotide identity and amino acid identity. These data suggested the genetic diversity of porcine sapoviruses in China.
Subject(s)
Animals , China , Genetic Variation , Genotype , Phylogeny , Sapovirus , Classification , Genetics , Sequence Homology, Nucleic Acid , Swine , VirologyABSTRACT
<p><b>OBJECTIVE</b>To detect human parechovirus (HPeV) from stool samples of hospitalized children for acute gastroenteritis of undetectable etiology.</p><p><b>METHODS</b>We conducted a real-time PCR to detect HPeV.</p><p><b>RESULT</b>The results showed that 24 of 99 (24%) children with gastroenteritis of undetectable etiology were detected with HPeV. Four known HPeV types (HPeV1, 3, 4, 6) were detected in the present study. HPeV1 (50%) was frequently identified as the predominant strain and follow by HPeV3 (25%), HPeV4 (8.3%) and HPeV6 (4.2%). We were unable to type 3 samples.</p><p><b>CONCLUSION</b>HPeV was prevalent in hospitalized children for acute gastroenteritis of undetectable etiology in China. Further study is needed for clarifying the role of HPeV in gastroenteritis.</p>
Subject(s)
Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Feces , Virology , Gastroenteritis , Virology , Molecular Sequence Data , Parechovirus , Classification , Genetics , Phylogeny , Picornaviridae Infections , VirologyABSTRACT
Two Rotavirus G9P[8] strains (LL52696 and LL52727) were recognized during a sentinel-based survey in Lulong, China. Phylogenetic analysis of the VP7 gene showed that both strains isolated constituted a divergent genetic cluster distinct from the other G9 strains isolated in China. Analysis of VP4, VP6, and NSP4 genes revealed that these strains were closely related to Lulong strains. We hold that two strains were reassortant between G9 and Lulong predominant strains.
Subject(s)
Humans , Amino Acid Sequence , Antigens, Viral , Chemistry , Genetics , Base Sequence , Capsid Proteins , Chemistry , Genetics , Glycoproteins , Chemistry , Genetics , Phylogeny , Rotavirus , Classification , Genetics , Toxins, Biological , Chemistry , Genetics , Viral Nonstructural Proteins , Chemistry , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To analyze epidemiological characters of an outbreak of rotavirus diarrhea in Daxing County, Guangxi Province.</p><p><b>METHODS</b>Rotavirus-positive specimens were identified by ELISA kit. G/P typing assays were confirmed with multiplex seminested RT-PCR. Full-length VP7 genes of 4 positive specimens were amplified and analyzed.</p><p><b>RESULTS</b>30 cases of Rotavirus-positive were identified from 64 specimens. The attack rate was 46.9%, and G/P typing was G1P[8]. A change of VP7 amino acid residue is at positions 68.</p><p><b>CONCLUSION</b>G1P[8] rotavirus is the etiologic agents of this diarrhea outbreak. In addition, adults were included in this outbreak.</p>